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Image Search Results
Journal: PLoS ONE
Article Title: Effects of subconjunctival administration of anti-high mobility group box 1 on dry eye in a mouse model of Sjӧgren’s syndrome
doi: 10.1371/journal.pone.0183678
Figure Lengend Snippet: (A) BrdU staining showing no significant proliferative changes in CD3 + T cells or B220 + B cells. (B) Percentage of Th17 (CD3 + CD4 + IL-17 hi ) cells and Tc17 (CD3 + CD8 + IL-17 hi ) cells were not altered after treatment. (C) IFNγ-secreting T cells (CD3 + CD4 + IFNγ hi or CD3 + CD8 + IFNγ hi ) were not affected by treatment. Data are presented as mean ± standard error. (Th17, T helper cells secreting IL-17; Tc17, cytotoxic T cells secreting IL-17).
Article Snippet: Color conjugation combinations and gating strategies were as follows: (1) IFNγ, and IL-17A secreting cells; CD3-PerCP cy5.5 (eBioscience, 145-2C11), CD4-APC (eBioscience, GK1.5), CD8-PE cy7 (eBioscience, 53–6.7),
Techniques: BrdU Staining
Journal: PLoS ONE
Article Title: Effects of subconjunctival administration of anti-high mobility group box 1 on dry eye in a mouse model of Sjӧgren’s syndrome
doi: 10.1371/journal.pone.0183678
Figure Lengend Snippet: (A) No significant change in percentage of plasma cells (CD3 - B220 + CD138 + cells). (B) No change in IL-10-secreting B regulatory cells (CD3 - CD19 + B220 + IL-10 hi ). (C) No change in the level of anti-SSA (RO 60) antibodies after treatment. (D) No significant changes in inflammatory foci scores (> 50 lymphocytes/focus) among all groups. Data are presented as mean ± standard error.
Article Snippet: Color conjugation combinations and gating strategies were as follows: (1) IFNγ, and IL-17A secreting cells; CD3-PerCP cy5.5 (eBioscience, 145-2C11), CD4-APC (eBioscience, GK1.5), CD8-PE cy7 (eBioscience, 53–6.7),
Techniques: Hi-C
Journal: PLoS ONE
Article Title: Effects of subconjunctival administration of anti-high mobility group box 1 on dry eye in a mouse model of Sjӧgren’s syndrome
doi: 10.1371/journal.pone.0183678
Figure Lengend Snippet: (A) Fold changes in ILC3 percentage (CD3 - B220 - CD45 + IL-22 hi cells; NCR + or NCR - ILC3) showing a significant increase following 2 μg anti-HMGB1 treatment compared with control (Kruskal-Wallis test, PBS vs. 2μg anti-HMGB1, *p = 0.025). Fold increase in NCR - ILC3 percentage (CD3 - B220 - CD45 + NKp46 - IL-22 hi cells)(Kruskal-Wallis test, PBS vs. 2 μg anti-HMGB1, **p = 0.0142). (B) Increased IL-22 levels after 2 μg anti-HMGB1 treatment (Kruskal-Wallis test, PBS vs. 2 μg anti-HMGB1, *p = 0.025). (C) No change in percentage of CD3 + IL-22 hi cells (Th22 cells or γδ T cells) in draining lymph nodes. Data are presented as mean ± standard error. (D) Representative images of NCR - ILC3s (CD3 - B220 - CD45 + NKp46 - IL-22 hi cells) in PBS- and 2 μg anti-HMGB1-treated groups. NCR, natural cytotoxicity receptor.
Article Snippet: Color conjugation combinations and gating strategies were as follows: (1) IFNγ, and IL-17A secreting cells; CD3-PerCP cy5.5 (eBioscience, 145-2C11), CD4-APC (eBioscience, GK1.5), CD8-PE cy7 (eBioscience, 53–6.7),
Techniques: